UCLA-led study reveals the mechanism that helps malaria parasites take over human red blood cells

3D imaging by California NanoSystems Institute could lead to new targeted therapeutics

Electron Imaging Center for NanoMachines

EICN provides advanced electron imaging tools for applications ranging from materials science to structural biology

Titan Krios

This new cryo-electron microscope will be installed in the EICN core facility located on the B level of the new CNSI building and will be an integral part of the advanced electron imaging capability at UCLA. Detail information and specification of this instrument will be provided in the near future. The following text is an excerpt from FEI’s literature on their Titan line.

The FEI Titan™ family currently offers the Titan 80-300 kV instruments, the world’s most powerful, commercially-available transmission electron microscope (TEM). Since its release in 2005, the Titan has been widely acclaimed both for its ability to deliver ground-breaking results and for its superior product design. It has rapidly become the preferred S/TEM of leading researchers around the world, enabling discovery and exploration down to sub-Ångström resolution in both TEM and S/TEM modes. Titan Family Advantages and Capabilities Performing at the 32 nm node, FEI’s Titan 80-300 kv transmission electron microscope images nanostructures with the highest level of detail available today—down to the sub-Ångström level, providing critical data for process development and materials research on a highly stable, easy-to-use platform. Titan is the next generation of a unique 80-300 kV range microscope for corrector and monochromator technologies. The stability, performance and ease-of-use of the Titan transmission electron microscope enable corrected microscopy to be taken to the next level, where new discoveries on the structure and property relationships of materials become possible at ever-decreasing scales. The Titan system is poised to bring electron microscopy into a new era by expanding boundaries and assisting scientists and researchers to achieve ground-breaking results in nanoresearch. Awards Since its launch in 2005, the Titan transmission electron microscope has won four awards for its design, performance and innovation:

  • Innovative Product of the Year presented by the Oregon Tech Awards in the United States
  • 2005 iF Product Design Award
  • Top Products of 2005 by Technology Magazine
  • Greatest Hits of 2005 by Micro Magazine

Titan Krios Operation

The Titan Krios transmission electron microscope is open for usage for all internal and external users. Please email us if you are interested at EICNHELP@CNSI.UCLA.EDU.

Typical Operation of Titan Krios for first timers includes 3-4 hours of assisted usage (academic rate $125/hour, industry rate: $250/hour), in addition to the Titan Krios hourly rate. Please see here for our current recharge rates: http://www.eicn.ucla.edu/node/106

Assisted usage includes

  • Approximately 1 hour to load your sample*
  • Approximately 1-2 hours to set up the microscope including alignment and mapping
  • Approximately 1 hour to set up the Leginon system and other software (single particle or batch tomography).
  • If necessary, additional hours to train the user on the system. This is based on the user’s experience and familiarity with the system.
  • When the Titan Krios is ready, you can start collecting images and processing your data on your own.

*Remember to bring your prepared samples on grids to our lab or ship them here prior to your arrival. Email us at EICNHELP@CNSI.UCLA.EDU for the address.

Supplies provided by EICN will be charged at cost. (Carbon Film Grids: $5/each, Quantifoil Grids: $15 each, Auto Grids: $30 each, etc.)

For assistance in tomography data processing, please contact Wong Hoi Hui at wonghoihui@ucla.edu