From now through October 31, 2026, the internal academic rate for the G1 will be reduced from $70/hour to $55/hour, providing a cost-effective opportunity to advance high-resolution structural biology projects.

• Automated specimen handling system (the FEI Autoloader)
• Automated liquid nitrogen cooling system
• Gatan image filter (GIF) BioQuantum with K3 camera
• FEI Xplorer3D data acquisition and processing system
• SerialEM automatic data acquisition system (Version 4.0.26)
• CryoSPARC Live for data processing

The Titan Krios transmission electron microscope is open for usage for all internal and external users. Instructions to become a user can be found here: EICN Become a User. Training can be requested by emailing eicnhelp@cnsi.ucla.edu. If you do not receive a reply within 48 hours, you can email Matthew Mecklenburg (mmecklenburg@cnsi.ucla.edu) to follow up.

Typical Operation of Titan Krios for first-time users includes 3-4 hours of assisted usage, in addition to the Titan Krios hourly rate. Please see here for our current recharge rates: EICN Policy and Rates.

Assisted usage includes:

• Approximately 1 hour to load your sample*
• Approximately 1-2 hours to set up the microscope including alignment and mapping
• Approximately 1 hour to set up data collection software (single particle or tomography).
• If necessary, additional hours to train the user on the system. This is based on the user’s experience and familiarity with the system.
• When the Titan Krios is ready, you can start collecting images and processing your data on your own.

*Remember to bring your prepared samples on grids to our lab or ship them here prior to your arrival. Email us at eicnhelp@cnsi.ucla.edu for the address.

Supplies provided by EICN will be charged at cost. (Carbon Film Grids: $5/each, Quantifoil Grids: $15 each, Auto Grids: $30 each, etc.)
Additional consumable fees may apply (e.g., $30 per autogrid). Autogrid packs of 10 are available from EICN at cost.Cold Field Emission Gun (E-CFEG)