Electron Imaging Center for Nanosystems

EICN provides advanced electron imaging tools for applications ranging from materials science to structural biology

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AKTA pure 25 M1 Fast Performance Liquid Chromatography (FPLC) Instrument

Description: The AKTA pure 25 M1 Fast Performance Liquid Chromatography (FPLC) instrument is reserved for purification of proteins and protein complexes. Users may use the provided size exclusion gel filtration columns or connect their own columns to the instrument.

Training can be requested by emailing eicnhelp@cnsi.ucla.edu. If you do not receive a reply within 48 hours, you can email Matthew Mecklenburg (mmecklenburg@cnsi.ucla.edu) to follow up.

Key Features:

  • Computer software: UNICORN 7.1
  • Triple wavelength UV detector (170nm-700nm)
  • Conductivity detector
  • Inlet valve V9-IAB; Outlet valve V9-Os
  • Max Operating Pressure: 20 MPa (2900 PSI)
  • Flow Cell: 10 mm
  • Flow Rates: 0.001-25 ml/min (normal range), 0.01-50ml/min (column packing flow)
  • Available injection loops: 1.0ml, 0.5mL and 0.1mL
  • Available columns:
    o     Superose 6 Increase 3.2/300 (GE product number: 29091598)
    o     Superose 6 Increase 10/300 GL (GE product number: 29091596)
  • Available fraction collector racks: 50 ml tubes cassette, 15 ml tubes cassette, deep-well plate cassette (compatible with 24, 48 or 96 deep-well)

Rates: Internal Academia rate: $5/hour; External Academia rate: $5/hour; Industry rate: $10/hour