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Electron Imaging Center for NanoMachines

EICN provides advanced electron imaging tools for applications ranging from materials science to structural biology


The TF20 TEM is from the FEI TecnaiG2 Family, and is suitable for both cryoelectron microscopy and cryoelectron tomography. Its features include:

  • 200kV, Field emission gun (FEG).
  • Computer-controlled cryoET.
  • Liquid nitrogen stage.
  • suitable for both cryoelectron microscopy and cryoelectron tomography.

This electron microscope is equipped with a TWIN lens. In addition to these features, the TF20 is controlled by a dedicated computer running Windows XP. Images captured from the TF20 can be recorded on either film or the TIETZ F415MP CCD camera. This camera is 4K x 4K with a pixel size of 15 ?m, and has multiport readout which should decrease the time necessary for information transmission. The image processing programs installed are EMMENU4, EMMENU3, and the TIETZ Tomography Package.

Instructions for Tomography using FEI Batch Tomography and EMTools.
Basic Usage of FEI Batch Tomography Imaging

Instructions for using the TF20.
TF20 Manual (Last Version)

You are required to have at least 10 hours of previous experience on any modern FEI TEM with compustage in order to be qualified for TF20 training. Instructions to become a user can be found here: EICN Become a User. Training can be requested by emailing If you do not receive a reply within 48 hours, you can email Matthew Mecklenburg ( to follow up.

Rates: Internal Academia rate: $60/hour; External Academia rate: $85/hour; Industry rate: $120/hour