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Electron Imaging Center for NanoMachines

EICN provides advanced electron imaging tools for applications ranging from materials science to structural biology

Titan Krios G1 TEM

Description: The Titan Krios G1 TEM is a cryoEM instrument for biological specimens from FEI. This instrument is dedicated to high-resolution cryo-electron microscopy of single particles and cryo-electron tomography of thick specimens. This electron microscope is equipped with a C-TWIN lens.

Key features:

  • 300 kV
  • Field Emission Gun (S-FEG)
  • 3 Condenser Lens Parallel Beam Zoom
  • 1.4 Å resolution (information limit)
  • Computer-controlled compustage
  • +/- 70° double-tilt specimen stage
  • Cs = 2.7 mm
  • Cc = 2.7 mm
  • Automated specimen handling system (the FEI Autoloader)
  • Automated liquid nitrogen cooling system
  • Gatan image filter (GIF) BioQuantum with K3 camera
  • FEI Xplorer3D data acquisition and processing system
  • SerialEM automatic data acquisition system (Version 4.0.26)
  • CryoSPARC Live for data processing


Rates:
Internal Academia rate: $70/hour; External Academia rate: $95/hour; Industry rate: $140/hour
Note: Additional consumable charges may apply (e.g. $30/autogrid)

Titan Krios Operation

The Titan Krios transmission electron microscope is open for usage for all internal and external users. Instructions to become a user can be found here: EICN Become a User. Training can be requested by emailing eicnhelp@cnsi.ucla.edu. If you do not receive a reply within 48 hours, you can email Matthew Mecklenburg (mmecklenburg@cnsi.ucla.edu) to follow up.

Typical Operation of Titan Krios for first timers includes 3-4 hours of assisted usage (academic rate $125/hour, industry rate: $250/hour), in addition to the Titan Krios hourly rate. Please see here for our current recharge rates: EICN Policy and Rates.

Assisted usage includes

  • Approximately 1 hour to load your sample*
  • Approximately 1-2 hours to set up the microscope including alignment and mapping
  • Approximately 1 hour to set up the Leginon system and other software (single particle or batch tomography).
  • If necessary, additional hours to train the user on the system. This is based on the user’s experience and familiarity with the system.
  • When the Titan Krios is ready, you can start collecting images and processing your data on your own.

*Remember to bring your prepared samples on grids to our lab or ship them here prior to your arrival. Email us at EICNHELP@CNSI.UCLA.EDU for the address.

Supplies provided by EICN will be charged at cost. (Carbon Film Grids: $5/each, Quantifoil Grids: $15 each, Auto Grids: $30 each, etc.)