Electron Imaging Center for Nanomachines
Services
Services Available
Training on Electron Microscopes or supporting instruments
An experienced staff will provide the necessary training(s) on an EM or other EICN instruments available. After training, a user will be authorized to independently reserve and operate the machine. Please see our list of equipments available here.
For training inquiries, email eicnhelp@cnsi.ucla.edu for more information.
Assistance on Electron Imaging
An EICN staff can assist users in acquiring high quality image data with an electron microscope and help users with their complex electron imaging needs.
Please email eicnhelp@cnsi.ucla.edu for more information.
Computer Processing
Skilled staff can assist you to process your single particle or tomography data. Users can take advantage of the software available to produce 3D reconstructions of their samples.
Please contact Wong Hoi Hui, WongHoiHui@ucla.edu, for assistance.
Sample Preparation (e.g. embedding, sectioning, etc.)
Please contact the UCLA Brain Research Institute Electron Microscopy core facility or Sergey Ryazantsev, sryazant@ucla.edu, for assistance.
EM Tomography Services
| Services | Stages | Sub Stage | Rates |
| Stage 1 |
Basic sample evaluation (Prepare 2 negative stain grids and use T12 EM to check if sample is of good quality enough to continue with tomography)
|
$175.00 per service Cost includes T12 EM usage for 0.5 hours, 1 hour of assisted usage, 2 EM grids, and data collected
|
|
| Stage 2 | Basic tomography imaging* | (if it is a cryo sample) | $95.00 per cryo sample Cost includes plunger usage for 0.5 hour, 0.5 hours of assisted usage, grids and gold beads |
| (Use TF20 EM to collect tomography data) | Load sample and set up TF20 EM for imaging |
$307.50 per session |
|
| Batch tomography imaging |
$157.50 per tilt series set |
||
| Stage 3 |
Data processing** (Align tilt series and reconstruct 3D volume using IMOD) |
$250.00 per tilt series set Cost includes 2 hours of assisted usage on a workstation computer & data storage |
Cost includes plunger usage for 0.5 hour, 0.5 hours of assisted usage, grids and gold beads
(Use TF20 EM to collect tomography data)
Load sample and set up TF20 EM for imaging
$307.50 per session
Cost included TF20 EM usage for 1.5 hours and 1.5 hour of assisted usage
Batch tomography imaging
$157.50 per tilt series set
Cost includes TF20 EM usage for 1.5 hours and 0.25 hour assisted usage
Stage 3
Data processing**
(Align tilt series and reconstruct 3D volume using IMOD)
$250.00 per tilt series set
Cost includes 2 hours of assisted usage on a workstation computer & data storage
* Tilt step is 1 degree for negative stain samples and 2 degrees for cryo samples to avoid radiation damage
** Includes basic picture and video of reconstructed map from 3Dmod
Please email EICNHELP@CNSI.UCLA.EDU if you are interested.